Journal
SCIENTIFIC REPORTS
Volume 5, Issue -, Pages -Publisher
NATURE RESEARCH
DOI: 10.1038/srep15915
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Funding
- Wellcome Trust [091911]
- NIH-Oxford-Cambridge Scholars Program
- Medical Research Council [MR/K015869/1, MR/K01563X/1, MR/K01577X/1] Funding Source: researchfish
- MRC [MR/K015869/1, MR/K01563X/1, MR/K01577X/1] Funding Source: UKRI
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Three-dimensional structured illumination microscopy (3D-SIM) is a versatile and accessible method for super-resolution fluorescence imaging, but generating high-quality data is challenging, particularly for non-specialist users. We present SIMcheck, a suite of ImageJ plugins enabling users to identify and avoid common problems with 3D-SIM data, and assess resolution and data quality through objective control parameters. Additionally, SIMcheck provides advanced calibration tools and utilities for common image processing tasks. This open-source software is applicable to all commercial and custom platforms, and will promote routine application of super-resolution SIM imaging in cell biology.
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