Journal
SEXUAL PLANT REPRODUCTION
Volume 25, Issue 4, Pages 281-291Publisher
SPRINGER
DOI: 10.1007/s00497-012-0197-0
Keywords
Pistil; Two-dimensional gel electrophoresis; Proteomics; Soybean; Pollination
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Funding
- 100 talents program of Chinese Academy of Sciences
- National Key Technology RD Program [2011BAD35B06]
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Siphonogamy is a critical process in plant reproductive growth, during which numerous cell-cell interaction events occur between pistil and pollen. Previous studies in Solanaceae, Papaveraceae, and Brassicaceae focusing on pollen-stigma recognition in self-incompatible systems have provided many important views. In this study, we profiled the proteome in soybean mature pistils before and after pollination. Comparative analyses of two-dimensional gel electrophoresis maps from un-pollinated and pollinated pistils were conducted. The results showed that 22 proteins were increased and 36 proteins decreased after pollination. Functional categorization showed that most of them were metabolism- and redox-related proteins. The enhancement of primary metabolism, biosynthesis of pollen tube guidance compounds, and adjustment of redox homeostasis system might be helpful for a successful pollination. Quantitative reverse transcript-polymerase chain reaction analysis implied that the regulation of gene expression might happen at both transcriptional and posttranscriptional levels during pollination. This study will enhance our understanding of pollen-stigma interaction in plant sexual reproductive growth.
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