Integrated Approach for β-glucosidase Purification from Non-Clarified Crude Homogenate using Macroporous Cryogel Matrix

The monolithic cryogel matrix of polyacrylamide-co-allylglycidyl ether (6%) was synthesized at sub-zero temperature which showed high flow-rate and could easily transport the macromolecules due to the large pore size in the range of 30-110 mu m. Cu2+-iminodiacetic acid modified cryogel was further used for separation of histidine-tagged -glucosidase directly from the disrupted cell homogenate. Under the three repetitive runs, the average purification factor, percent recovery, and yield was approximately 2, 93%, and 80%, respectively. These results showed that continuous macroporous cryogel column can be potentially utilized for direct capture of labile products from suspensions and cell homogenate.