Journal
SEPARATION AND PURIFICATION TECHNOLOGY
Volume 138, Issue -, Pages 98-103Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.seppur.2014.10.001
Keywords
Raffinose family oligosaccharides; Rehmannia glutinosa Libosch.; FPLC-RID; GC-MS; LC-MS/MS
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Funding
- Science and Technology Development Fund of Macao [FDCT059/2011/A3]
- University of Macau [MYRG085, MYRG140]
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Raffinose family oligosaccharides (RFOs) were extracted and isolated from Rehmannia glutinosa Libosch. by using microwave-assisted extraction and fast protein liquid chromatography coupled with refractive index detection (FPLC-RID). After extraction with 60% ethanol (v/v) and decolorization with MCI gel CHP20P, total oligosaccharides were purified by using FPLC-RID coupled with the Bio-Gel P2 column eluted with water at the flow rate of 0.3 mL/min. Three pure oligosaccharides (DP3-DP5) were obtained with the yields of 1.72%, 7.52% and 0.51%, respectively, as well as identified as raffinose, stachyose and verbascose from the results of liquid chromatography coupled with tandem mass spectroscopy (LC-MS/MS) and gas chromatography coupled with mass spectroscopy (GC-MS) analysis. A large-scale of pure RFOs was automatically and efficiently separated from R. glutinosa by using FPLC-RID method, which was beneficial for the qualitative and quantitative analysis of RFOs in R. glutinosa and their products. (C) 2014 Elsevier B.V. All rights reserved.
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