Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 275, Issue -, Pages 199-205Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2018.08.010
Keywords
MoSe2 nanosheet; Hollow gold nanosphere; Squamous cell carcinoma antigen; Photoelectrochemical immunosensor
Funding
- National Natural Science Foundation of China [21675175, 21275166]
- Major Projects of Technical Innovation of Hubei Province [2017ACA172]
- Natural Science Foundation of Hubei Province [2018CFB617, 2015CFA092]
- National Higher-eduction Institution General Research and Development Project [3212015GZQ15003]
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A photoelectrochemical immunosensor was fabricated for squamous cell carcinoma antigen (SCCA) assay with high performances by using MoSe2 nanosheets and hollow gold nanospheres as photosensitive materials. MoSe2 nanosheets were simply synthesized with a hydrothermal method using 1,3-di-(3-thioacetylpropyl)-imidazolium bromide (DITAPIBr) ionic liquid as a morphological controlling reagent, selenium powder, and sodium molybdate as raw materials. Hollow gold nanospheres (HGNs) were prepared by using 1,3-di-(3-bromopropyl)-imidazolium bromide ionic liquid (DIBPIBr) as functional monomer, and NaBH4 as a reductant to interact with the HAuC1(4 )solution. Subsequently, HGNs were integrated with MoSe 2 nanosheets to provide a HGNs-MoSe(2 )nanocomposite. The photocurrent intensity of MoSe2 nanosheets was significantly enhanced by the hybridization of HGNs. Furthermore, HGNs-MoSe2 nanocomposites were coated onto a glassy carbon electrode surface to construct a platform for the immobilization of SCCA antibody (anti-SCCA), resulted in a photoelectrochemical immunosensing system for determining SCCA. Under optimized conditions, the fabricated immunosensor shows high selectivity and sensitivity towards SCCA, and exhibits a detection limit of 0.21 pg mL(-1) and a linear range from 1.0 pg mL(-1) to 50 ng mL(-1). Meanwhile, the practicability of the immunosensor was demonstrated by determining SCCA in clinical serum samples with high accuracy.
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