Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 274, Issue -, Pages 338-342Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2018.07.167
Keywords
Affinity assay; Biosensor; Nanofluidics
Funding
- Agence Nationale de la Recherche [ANR-13-IS10-0001]
- Ministry of Science and Technology, Taiwan [103-2923-M-001-007-MY3]
- MAE (Ministere des Affaires Etrangeres)
- French RENATECH Network
- Academia Sinica Integrated Thematic Project [AS-106-TP-A03]
- AOARD grant [134023]
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We report a simple and cost-effective nanofluidic fluorescence microscopy platform with parallel kinetic assay capability for the determination of kinetic parameters in a single run. An on-chip microfluidic concentration diluter, or gradient generator, was integrated to a biofunctionalized nanofluidic chip, enabling simultaneous interrogation of multiple biomolecular interactions with a full titration series of analyte in a single experiment. We demonstrate that since the association and dissociation phases are induced by the on-chip gradient generator and a reverse buffer flow operation, complete kinetic sensorgrams for IgG/anti-IgG interactions can be achieved within 20 min on a single device, which is at least 10 times faster than traditional kinetic techniques. This method could contribute to low-cost, rapid and high-throughput drug-screening and clinical diagnostics.
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