Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 274, Issue -, Pages 349-355Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2018.07.122
Keywords
Photoelectrochemical immunosensor; Dual-inhibition effect; CeO2-CdS; SiO2/PDA-Ag NPs; BNP
Funding
- National Natural Science Foundation of China [21627809, 21575050, 21505051]
- Special Foundation for Taishan Scholar Professorship of Shandong Province
- UJN [ts20130937]
- Foundation of Key Laboratory of Sensor Analysis of Tumor Marker, Ministry of Education, Qingdao University of Science and Technology
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A novel sandwich photoelectrochemical (PEC) immunosensor was constructed for ultrasensitive brain natriuretic peptide (BNP) detection. In this work, CeO2-CdS was employed as excellent photoactive sensing platform, and SiO2/PDA-Ag nanocomposite was used as signal amplification element. CeO2-CdS, providing great photocurrent response due to the well matched energy band which effectively impeded the recombination of the electrons and holes. For SiO2, due to the poor electro-conductivity and easy modification, it could be well used as an excellent signal-off label. For the sake of improving sensitivity of the immunosensor, Ag nanoparticles (Ag NPs) was applied owing to the energy transfer between CdS, which could decrease the PEC response obviously for amplifying the change of signal value. Besides, Ag NPs could anchor plenty of the detection antibodies by the chemical bonding between Ag NPs and -NH2 of antibodies. Using PDA to connected SiO2 and Ag NPs because of it great biocompatibility and reducing capacity. The well-established PEC immunosensor exhibited a low detection limit of 0.05 pg mL(-1) (S/N= 3) with a linear range from 0.1 pg mL(-1)-5 ngmL(-1) for BNP detection. In addition, the immunosensor also exhibited good stability, satisfactory reproducibility and acceptable specificity, which could be applied to detect other biomarkers.
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