A novel ECL biosensor based on C60 embedded in tetraoctylammonium bromide for the determination of glucose

A highly sensitive electrochemiluminescence (ECL) biosensor for glucose detection has been developed through immobilizing glucose oxidase (GOD) on a glassy carbon electrode (GCE) modified with C-60 embedded in tetraoctylammonium bromide (TOAB(+)) film. C-60 is a promising electron transfer mediator between GOD and GCE surface. Meanwhile, C-60 has excellent catalytic performance to directly enhance the ECL intensity of luminol. In addition, the introduction of C-60 and TOAB(+). can immobilize more enzymes effectively onto the electrode so that they can enhance the sensitivity and detection limit of ECL biosensor. Under the optimum condition, the developed glucose biosensor exhibited the linear response range from 500 nM to 13 mM with a detection limit of 166.7 nM (at the signal-to-noise ratio of 3). The biosensor offers an alternative analytical method with excellent properties of high selectivity, long-term stability and outstanding reproducibility. (C) 2014 Elsevier B.V. All rights reserved.