Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 200, Issue -, Pages 304-309Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2014.04.030
Keywords
Dialdehyde cellulose film; Antibody immobilization; Electrochemical immunosensor; Human immunoglobulin G
Funding
- construct program of the key discipline in Hunan province (Applied Chemistry)
- Scientific Research Fund of Hunan Provincial Education Department [13C635]
- Startup Foundation for Doctors of Hunan University of Arts and Science [10305005]
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Antibodies can be directly immobilized on the surface of dialdehyde cellulose film through the covalent bonding of the aldehyde groups of the dialdehyde cellulose with amino groups of antibodies. In this work, human IgG was used as a model analyte to fabricate an electrochemical immunosensor. Using the proposed immunosensor, we detected IgG within the range from 0.5 to 45 ng/mL with a detection limit of 0.3 ng/mL (S/N = 3). The electrochemical immunosensor had a good specificity, stability and reproducibility. This strategy may pave a simple way to fabricate an electrochemical immunosensor in other applications. (C) 2014 Elsevier B.V. All rights reserved.
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