Electrochemical sensor for bovine hemoglobin based on a novel graphene molecular imprinted polymers composite as recognition element

A highly selective and sensitive electrochemical sensor for bovine hemoglobin (BHb) was developed basedon a novel graphene-molecular imprinted polymers composite (GR-MIP) as recognition element. The GRMIP composite was synthesized by oxide self-polymerization of dopamine on the surface of graphene inthe presence of template molecules (BHb) in aqueous solution, which is a one-pot facile and green preparation procedure. The fabrication process of the GR-MIP sensor was characterized by differential pulsevoltammetry (DPV) and electrochemical impedance spectroscopy (EIS), in which [Fe(CN)(6)](3-/4-) was usedas an electrochemical active probe. The as-prepared GR-MIP sensor was tested by DPV to investigate therelationship between the current response and BHb concentration. Under optimized experimental conditions, selective detection of BHb in a concentration range of 1.0 x 10(-9)mg mL(-1)to 1.0 x 10(-1)mg mL(-1)with detection limit of 2.0 x 10-10mg mL(-1)was obtained, which outperformed the most of reported BHbdetecting methods. This novel GR-MIP sensor also exhibited excellent selectivity and good reproducibility, and was used to the determination of BHb in real samples with satisfactory results. The outstandingsensing behavior of the target protein coupled to its low production cost and facile, green preparationprocedure makes the GR-MIP sensor attractive in the sensitive and selective detection of target proteinsin biological fluids. (C) 2014 Elsevier B. V. All rights reserved.