Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 196, Issue -, Pages 203-207Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2014.01.121
Keywords
Hypochlorous acid; Fluorescent probe; Coumarin; Bioimaging
Funding
- National Science Council (ROC)
- National Chiao Tung University
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A coumarin-based fluorometric probe, HS1, has been successfully developed for highly sensitive and selective detection of hypochlorous acid (HOCl). The probe is based on the specific HOCl-promoted oxidation of oxime. The reaction is accompanied by a 7-fold increase in the fluorescent quantum yield (from 0.03 to 0.21). The fluorescence intensity of the reaction between HOCl and HS1 shows a good linearity in the HOCl concentration range of 1-20 mu M and a low detection limit of 25 nM (S/N = 3). In addition, confocal fluorescence microscopy imaging using RAW264.7 macrophages demonstrates that the HS1 probe could be an efficient fluorescent probe for HOCl in living cells. (C) 2014 Elsevier B.V. All rights reserved.
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