Simple fabrication of ZnO/Pt/chitosan electrode for enzymatic glucose biosensor

Enzymatic glucose biosensor was fabricated with the aid of zinc oxide (ZnO) porous nanostructure on FTO glass substrate by simple drop casting technique. By introducing a small layer of platinum (Pt) nanopartides and chitosan (CS) over the ZnO porous nanostructure have enhanced the glucose sensing property of the fabricated biosensor to a higher order. The morphological and structural properties of the electrodes were analyzed using scanning electron microscopy and X-ray diffraction analysis. The glucose sensing properties of glucose oxidase (GOx) immobilized electrodes were estimated using electrochemical analysis. The ZnO/Pt/CS heterostructure conjugated with GOx shows good analytical performances with high sensitivity (62.14 mu A mM(-1) cm(-2)) in a wide linear range and low detection compared with the bare ZnO/CS/GOx biosensor. In addition, the catalytic properties of the electrodes in H2O2 solution was studied and discussed in detail using cyclic voltammetry and impedance spectroscopic techniques. (C) 2014 Elsevier B.V. All rights reserved.