Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 201, Issue -, Pages 567-571Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2014.05.044
Keywords
Amyloid-beta 1-42; Alzheimer's disease; Screen Printed Carbon Electrode; Electrochemical immunosensor; Gold nanoparticles
Funding
- Spanish Ministry of Science and Innovation Project [MICINN-12-CTQ2011-24560]
- Severo Ochoa Predoctoral Grant [BP11-097]
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Alzheimer's disease is the most common form of dementia, characterized by the progressive accumulation of plaques with amyloid-beta peptide of 42 amino acids as one of the primary constituents. A disposable electrochemical immunosensor for the detection of amyloid-beta 1-42 is developed. Screen-Printed Carbon Electrodes nanostructured with gold nanoparticles generated in situ are used as the transducer surface. The immunosensing strategy consists in a competitive immunoassay: biotin-amyloid-beta 1-42 immobilized on the electrode surface and the analyte (amyloid-beta 1-42) compete for the anti-amyloidbeta 1-42 antibody. The electrochemical detection is carried out using an alkaline phosphatase labeled anti-rabbit IgG antibody. The analytical signal is based on the anodic stripping of enzymatically generated silver by cyclic voltammetry. The immunosensor achieved shows a low limit of detection (0.1 ng/mL) and a wide linear range (0.5-500 ng/mL). (C) 2014 Elsevier B.V. All rights reserved.
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