Journal
SCIENTIFIC REPORTS
Volume 5, Issue -, Pages -Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/srep12335
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Funding
- KU Endowment
- National Cancer Institute [P50-CA136393]
- Department of Defense Ovarian Cancer Research Program [W81XWH-10-1-0386, W81XWH-10-1-0525]
- Frontiers: The Heartland Institute for Clinical and Translational Research
- CTSA grant
- UL1TR000001
- Kansas Intellectual and Developmental Disabilities Research Center [HD002528]
- Illumina
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Current genomic studies are limited by the availability of fresh tissue samples. Here, we show that Illumina RNA sequencing of formalin-fixed diagnostic tumor samples produces gene expression that is strongly correlated with matched frozen tumor samples (r > 0.89). In addition, sequence variations identified from FFPE RNA show 99.67% concordance with that from exome sequencing of matched frozen tumor samples. Because FFPE is a routine diagnostic sample preparation, the feasibility results reported here will facilitate the setup of large-scale research and clinical studies in medical genomics that are currently limited by the availability of fresh frozen samples.
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