Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 191, Issue -, Pages 681-687Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2013.10.054
Keywords
Biosensor; Carbofuran; Iron oxide-chitosan nanocomposite; Acetylcholinesterase; Carbamate pesticide
Funding
- University Grants Commission, New Delhi, Govt. of India under the University with Potential for Excellence Scheme to Madurai Kamaraj University
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We report a highly sensitive square wave voltammetric biosensor for the determination of carbofuran using an acetylcholinesterase (AChE) enzyme immobilized iron oxide-chitosan nanocomposite film modified glassy carbon electrode (AChE/Fe3O4-CHIGCE). The Fe3O4-CH nanocomposite was prepared by a simple solution mixing process and its formation was confirmed by FT-IR spectroscopy. The effective enzyme immobilization onto the nanocomposite matrix was confirmed by electrochemical impedance, scanning electron and atomic force microscopy studies. Various experimental parameters such as effect of scan rate, inhibition time and substrate concentration were optimized. The nanocomposite-based biosensor could detect carbofuran as low as 3.6 x 10(-9) M. The reproducibility of the AChE/Fe3O4-CHIGCE was ascertained by performing intra-assay and inter-assay experiments using cyclic voltammetry. The practical application of the biosensor was ascertained by the determination of carbofuran from cabbage samples and by comparing the results with those obtained by the standard high-performance liquid chromatography (HPLC) method. (C) 2013 Elsevier B.V. All rights reserved.
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