A label-free electrochemiluminescence immunosensor based on silver nanoparticle hybridized mesoporous carbon for the detection of Aflatoxin B1

A novel electrochemiluminescence (ECL) immunosensor was fabricated for the detection of Aflatoxin B-1 (AFB(1)) by luminol functionalized with silver nanoparticles which were loaded on mesoporous carbon (luminol-AgNPs@MC) as antibody carriers and sensing platform. The immunosensor was prepared by modifying luminol-AgNPs@MC which conjugated AFB(1) antibody (anti-AFB(1)) on glassy carbon electrode. The use of single-step cycle pulse can limit the consecutive reaction between luminol and H2O2 and can be efficient to keep luminol ECL intensity. This proposed method is simple, fast, sensitive, specific, stable and reliable. The results show that the ECL immunosensor has high sensitivity for AFB(1) with a linear range from 0.1 pg/mL to 50 ng/mL and a low detection limit of 50 fg/mL. This work reveals that the luminolAgNPs@MC is excellent sensing platform for the fabrication of simple and sensitive immunosensors. Our study provided a promising technique for the quality inspection of agricultural products. (C) 2014 Elsevier B.V. All rights reserved.