Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 201, Issue -, Pages 360-368Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2014.05.015
Keywords
Immunosensor; Reduced graphene oxide carried thionine; Reduced graphene oxide-carried prussian blue; Aloe like gold microstructures; Simultaneous detection
Funding
- National Natural Science Foundation of China [20675002]
- Young Foundation of Wannan Medical College [WK201210]
- Natural Science Foundation of Anhui province of China [1208085QE102]
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In this work, we designed a novel sandwich-type electrochemical immunosensor for simultaneous detection of carcinoembryonic antigen (CEA) and alpha-fetoprotein (AFP) using non-enzymatic multiple-label method. Firstly, gold nanoparticles (AuNPs) decorated reduced graphene oxide (rG0)-carried prussian blue (PB) and AuNPs decorated rGO-carried thionine (Thi) (denoted as AuNPs-PB@rGO and AuNPsThi@rGO, respectively) were employed as distinguishable signal tags, which were utilized to load anti-CEA (Abm) and anti-AFP (Ab2,2), respectively. Subsequently, hierarchically aloe-like gold microstructures (HAG) with large effective area were used to immobilize the capture antibodies anti-CEA (Abi,i) and anti-AFP (Ab1,2), which effectively enhanced the performance of the immunosensor. Experimental results revealed that the sandwich-type immunosensor can permit the simultaneous detection of CEA and AFP with linear range of 0.6-80 ngmL-1 for two analytes, the detection limit was 0.12 ngmL-1 for CEA and 0.08 ngmL-1 for AFP (at a signal to noise ratio of 3), respectively. The immunosensor was applied to real sample analysis, and the recovery was within 95.9%-105.6% for CEA and 95.6%-105.4% for AFP. In addition, the immunosensor exhibited good reproducibility and stability, suggesting the immunosensor offered potential in analysis of biological samples. (C) 2014 Elsevier B.V. All rights reserved.
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