Potentiality of chemometric approaches for the determination of (+)-catechin in green tea leaves at the surface of multiwalled carbon nanotube paste electrode

Differential pulse voltammetry technique assisted by chemometric methods such as multivariate curve resolution-alternating least squares (MCR-ALS) has been proposed as a valuable approach for (+)-catechin determination in the presence of gallic acid at the surface of multiwalled carbon nanotube paste electrode. Central composite design and response surface methodology were used to optimize the influencing parameters. To determine (+)-catechin in the presence of unexpected electroactive interference with a very high degree of overlapping, second-order electrochemical data were generated by changing the pulse height as an instrumental parameter. After potential shift correction, MCR-ALS results show that second-order calibration could be applied with great success for electroanalytical determination of highly overlapped electroactive species. The linear least-squares calibration curve based on the area under concentration profile was provided over the range of 0.10-2.69 mu M for (+)-catechin, whereas detection limit was found to be 0.017 mu M. Also in this study, the effect of rotational ambiguity associated with a particular MCR solution under a set of constraints was investigated. (C) 2013 Elsevier B.V All rights reserved.