Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 178, Issue -, Pages 176-184Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2012.12.071
Keywords
Mach-Zehnder interferometer; Michelson interferometer; Intermodal; Fiber-optic immunosensor; Label free; Waist-enlarged fusion taper
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Label-free, all-fiber immunosensors based separately on Mach-Zehnder interferometry (MZI) and Michelson interferometery (MI) implemented using only single mode fiber (SMF) are proposed and experimentally demonstrated. The proposed SMF-MZI and SMF-MI sensors rely on intermodal interference between the core mode and cladding modes to detect ambient refractive index changes due to protein absorption on the cladding-ambient interface. The fiber surfaces were functionalized with self-assembled polyelectrolyte layers (chitosan (CS)/polysodium styrene sulfonate (PSS)). Immunoglobulin G (IgG) was immobilized on the polyelectrolyte layer and anti-immunoglobulin G (anti-IgG) molecular binding events were monitored through measurement of wavelength shifts. The proposed immunosensors exhibit anti-IgG detection sensitivities of 27.37 nm/(ng/mm(2)) and 5.91 nm/(ng/mm(2)) with concentration detection limits of 0.181 nM and 4.941 nM for MZI and MI sensor respectively. The specificities of the sensors were investigated using correlated/non-correlated anti-IgG-IgG pairs. These results demonstrate the feasibility of these sensors for various bio/chemical applications such as DNA hybridization detection and immunosensing. (c) 2012 Elsevier B.V. All rights reserved.
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