Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 185, Issue -, Pages 258-264Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2013.04.040
Keywords
Aflatoxin B-1; Multiwalled carbon nanotubes; Electrochemical detection; Electrophoretic deposition
Funding
- DST
- CSIR, India
- Department of Science and Technology, India [DST/TSG/ME/2008/18, GAP-081132]
- Indian Council of Medical Research, India [ICMR/5/3/8/91/GM/2010-RHN]
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We report results of studies relating to the development of an electrochemical immunosensor based on carboxylated multiwalled carbon nanotubes (c-MWCNTs) electrophoretically deposited onto indium tin oxide (ITO) glass. This c-MWCNTs/ITO electrode surface has been functionalized with monoclonal aflatoxin B-1 antibodies (anti-AFB(1)) for the detection of aflatoxin-B-1 using electrochemical technique. Electron microscopy, X-ray diffraction and Raman studies suggest successful synthesis of c-MWCNTs and the Fourier transform infra-red spectroscopic (FT-IR) studies reveal its carboxylic functionalized nature. The proposed immunosensor shows high sensitivity (95.2 mu A ng(-1) mL cm(-2)), improved detection limit (0.08 ng mL(-1)) in the linear detection range of 0.25-1.375 ng mL(-1). The low value of association constant (0.0915 ng mL(-1)) indicates high affinity of immunoelectrode towards aflatoxin (AFB(1)). (C) 2013 Elsevier B.V. All rights reserved.
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