Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 188, Issue -, Pages 227-234Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2013.07.028
Keywords
Hemoglobin; Blend; Biosensor; Electrocatalysis; Hydrogen peroxide
Ask authors/readers for more resources
In this study, we investigate the direct electron-transfer reactivity of immobilized hemoglobin (Hb) on a biocompatible poly(styrene-alternative-maleic acid) (PSMAC) copolymer/multi-walled carbon nanotubes (MWCNTs) blend for biosensor designs. The copolymer possesses good biocompatibility, uniformity, conformability and is ready for protein immobilization. On the other hand, electrochemical measurements show that the presence of MWCNTs provides a unique structure for interaction of Hb and copolymer and facilitate the direct electron transfer between the immobilized Hb and the surface conductivity. The immobilized Hb maintains its bioactivities and displays an excellent electrochemical behavior. The proposed method opens a way to develop biosensors by using nanostructured materials mixed with low electrical conductivity matrixes. The biosensor was used to catalyze the reduction of hydrogen peroxide. The electrocatalytic response showed a linear dependence on the H2O2 concentration ranging widely from 1.0 to 56.0 mu M with a detection limit of 0.38 mu M at 3 sigma. (C) 2013 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available