Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 176, Issue -, Pages 698-703Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2012.10.006
Keywords
Iminofluorescein-Cu2+ ensemble; Fluorescence; Sensor; Thiols; Biological imaging
Funding
- NSFY of China [21002049]
- Natural Science Foundation of the Jiangsu Higher Education Institutions of China [10KJB150005]
- Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry
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An iminofluorescein-Cu2+ ensemble probe was prepared for the detection of thiols in aqueous solution. In HEPES buffer, among various amino acids, only cysteine (Cys) induced fluorescence enhancement of Cu2+ ensemble. The mechanism was proposed that addition of Cys induced decomplexation of Cu2+ from weak-fluorescent ensemble, followed by hydrolytic cleavage of the resulted Schiff base to give a strongly fluorescent fluoresceinaldehyde. By fluorescence titration using 2 mu M iminofluorescein-Cu2+ ensemble probe, the detection limit for Cys was evaluated to be 9 mu M with a signal-to-noise ratio of 3. Furthermore, the confocal microscopy experiments demonstrated that this sensor was potential for the imaging of thiols in living cells. (C) 2012 Elsevier B.V. All rights reserved.
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