Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 181, Issue -, Pages 575-583Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2013.02.050
Keywords
Bienzyme biosensor; Carbon nanotubes; Gold nanoparticles; Horseradish peroxidase; Cholesterol oxidase
Funding
- National Natural Science Foundation of China [30800247, 20805043]
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A bienzyme biosensor for the detection of cholesterol, based on functionalized carbon nanotubes (CNTs) was fabricated through layer-by-layer (LBL) assembly technique. Being wrapped with cationic poly(allylamine hydrochloride) (PAH), the nanocomposite, multiwalled carbon nanotubes (MCNTs) mixed with gold nanoparticles (GNPs) was water soluble and positively charged. Based on reliably electrostatic interaction, bienzyme biosensor was then fabricated by LBL assembly of the positively charged nanocomposite and the negatively charged enzymes, horseradish peroxidase (HRP) and cholesterol oxidase (ChOx). The fabricated process and the electrochemical behaviors of the biosensor were investigated by scanning electron microscopy (SEM), electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV). Under the optimal conditions, the bienzyme biosensor obtained the linear range from 0.18 to 11 mM, with a detection limit of 0.02 mM. The proposed biosensor exhibited good sensitivity, stability, and controllability for the detection of cholesterol. (c) 2013 Elsevier B.V. All rights reserved.
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