Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 177, Issue -, Pages 634-642Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2012.11.028
Keywords
Magnetic nanoparticles decorated MWCNTs; Hydrodynamic amperometry; Guanine and adenine
Funding
- Isfahan University of Technology Research Council
- Center of Excellence for Sensor and Green Chemistry
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An electrochemical sensor was developed for guanine and adenine detection using multiwall carbon nanotubes (MWCNTs) decorated with NiFe2O4 magnetic nanoparticles on a glassy carbon electrode. Incorporation of MWCNT/NiFe2O4 nanohybrid on the surface of the electrode significantly increases oxidation the peaks currents but reduces the peaks potential of guanine and adenine. The modified electrode was employed for detecting purine bases using linear sweep voltammetry and hydrodynamic amperometry. With hydrodynamic amperometry, the peaks current of both guanine and adenine showed linear dependence on their concentrations in the range of 3.0-45.0 mu mol L-1 for guanine and 3.0-40.0 mu mol L-1 for adenine. When linear sweep voltammetry was used, the peak currents exhibited linear dependence on guanine in the concentration range of 0.05-3.0 mu mol L-1 and on adenine in the concentration range of 0.1-4.0 mu mol L-1. The limit of detection (S/N = 3) was found to be 0.006 and 0.01 mu mol L-1 for guanine and adenine, respectively. Linear sweep voltammetry was also used for simultaneous determination of adenine and guanine. Finally, the proposed electrochemical sensor was employed to determine guanine and adenine in single-strand deoxyribonucleic acid (ssDNA) samples with satisfactory results. (c) 2012 Elsevier B.V. All rights reserved.
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