Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 182, Issue -, Pages 696-705Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2013.03.030
Keywords
Long-term cell viability; ECIS; Lab-on-a-chip
Funding
- US Army Research Office [W911NF-10-1-0150]
- US Army Research Office
- MIT-ISN HBCU-MI
- U.S. Army Center of Environmental Health Research (USACEHR) [W81XWH-11-C-0026]
- Directorate For Engineering
- Div Of Electrical, Commun & Cyber Sys [1337965] Funding Source: National Science Foundation
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Biosensors that employ live mammalian cells as sensing elements require precise information about the cell longevity. These biosensors could be stored in an incubator and used during the lifetime of the cells. This paper is a study of the longevity of bovine aortic endothelial cells (BAECs) that are used as sensorial component for cell-based biosensors. Different types of polydimethylsiloxane (PDMS) cell culturing chambers along with the culturing conditions required for BAECs to survive long term in lab on a chip systems are presented. The electric cell-substrate impedance (ECIS) technique was used to monitor cell viability over extended time periods. Media was automatically recirculated over the cells by a portable pump, in order to create the conditions required for testing the sensor in the field. It was demonstrated the BAECs could survive up to 37 days. (C) 2013 Elsevier B.V. All rights reserved.
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