Two novel fluorescein-based fluorescent probes for hypochlorite and its real applications in tap water and biological imaging

Two novel fluorescein-based OCl- chemosensors were designed, synthesized and characterized. Both fluorescence probes utilize an irreversible OCl--promoted oxidation reaction to trigger the activation of fluorescence. These probes were shown to be highly selective for OCl-, and showed real time responses as well as a positive linear relationship to OCl- concentrations. The probes were successfully applied for the detection of OCl- in tap water and for fluorescence imaging of OCl- in bacterial Rhodobacter ferrooxidans sp. strain SW2 cell-EPS-mineral aggregates. (C) 2013 Elsevier B.V. All rights reserved.