Electrochemical sensor for determination of desipramine in biological material

A simple electrochemical method for determination of desipramine in human oral fluid was developed. Two types of electrodes, namely: glassy carbon electrode (GCE) and screen-printed electrode (SPE) were tested with different electrochemical techniques: cyclic voltammetry (CV) and differential pulse voltammetry (DPV). The simplex method was applied to find optimal instrumental conditions of DPV measurements. Three parameters were subjected to optimization with the simplex method procedure, namely: potential of the pulse (E-p), potential difference between pulses (E-s) and pulse time (t(p)). Buffer solution pH and material of the working electrode were tested in a pre-optimization step. For optimal working conditions of a given screen-printed electrode two ranges of desipramine concentration were tested. Signals registered for desipramine within the range of 0.01-1 mu M enabled its determination in alternative biological material such as oral fluid. (C) 2012 Elsevier B.V. All rights reserved.