Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 176, Issue -, Pages 386-389Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2012.10.054
Keywords
TiO2 nanoparticles; Carbon ionic liquid electrode; DNA biosensor; Electrochemical impedance spectroscopy; PML/RARA fusion gene
Funding
- National Natural Science Foundation of China [21205057, 21101087, 20975057]
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A high-performance DNA hybridization biosensor was constructed with nanosized TiO2 supported carbon ionic liquid electrode (CILE) as the sensing interface. The morphology of TiO2 nanoparticles and electrochemical performance of the TiO2/CILE were systematically investigated. The decoration of CILE with nanosized TiO2 integrated the advantages of nanosized TiO2 and CILE, including large surface area, excellent conductivity, and fine biocompatibility, which provided a favorable platform for DNA detection. Remarkable change has been observed in the impedance spectra before and after hybridization of probe ssDNA with the target DNA under optimal conditions. The sequence-specific DNA of PML/RARA fusion gene from acute promyelocytic leukemia could be quantified in a wide range of 1.0 x 10(-13) to 1.0 x 10(-8) mol/L with a detection limit of 2.8 x 10(-14) mol/L. The approach does not need oligonucleotide probe or target to be labeled previously, which makes it advantageous in terms of simplicity and nonin-vasiveness. (C) 2012 Elsevier B.V. All rights reserved.
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