Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 181, Issue -, Pages 439-445Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2013.02.030
Keywords
Dielectrophoresis; Impedance measurement; Bacteria detection; Trajectory
Funding
- Japan Society for the Promotion of Science [20360184, 21651063, 22110510]
- Grants-in-Aid for Scientific Research [25820174, 21651063, 23760370, 20360184, 22110510] Funding Source: KAKEN
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In this study, a bacterial detection technique and device that utilizes advantages of both positive and negative dielectrophoresis (DEP) has been proposed and demonstrated. The device has two microelectrodes, which serve as a bacteria concentrator using negative DEP (n-DEP) and as a bacteria detector using positive DEP (p-DEP), respectively. Bacteria flowing into the device are repelled under action of n-DEP force exerted by the first microelectrode, and are pushed toward the second microelectrode situated at the downstream. Then concentrated bacteria are finally captured by p-DEP on the second microelectrode and detected by dielectrophoretic impedance measurement (DEPIM) method. The numerical simulations and experiments proved that n-DEP concentrator could make DEPIM sensitivity two times higher than that without n-DEP as a result of increased number of bacteria trapped on the p-DEP microelectrode. (C) 2013 Elsevier B.V. All rights reserved.
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