Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 168, Issue -, Pages 223-230Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2012.04.012
Keywords
Immunosensor; Aflatoxin M1; Silver wire electrode; Electrochemical impedance spectroscopy; Label-free detection; Milk
Funding
- National Agriculture Innovation Project (NAIP) [C4/C10125]
- ICAR
- World Bank
- NAIP for the award of Research Associate Fellowship
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A highly sensitive and selective label-free impedimetric immunosensor based on silver (Ag) wire electrode for the detection aflatoxin M1 (AFM1) in milk is presented. The sensor was comtructed by functionalizing Ag wire coupled with selective monoclonal antibodies of AFM1 through sell assembled monolayers. The antigen-antibody interaction was quantified by measuring impedance in the frequency range (1-100 Hz) at 10 mV applied ac potential. A linear working range (6.25-100 pg mL(-1)) for AFM1 was obtained with 20 min analysis time. The sensor sensitivity was found to be about 2.1% impedance change per decade with limit of quantitation 6.25 pg mL(-1) and limit of detection 1 pg mV. Good recoveries were obtained with SD = 1.13 and R-2 = 0.982 in spiked milk samples. The proposed method is useful for sensitive analysis of AFM1 in milk. (C) 2012 Elsevier B.V. All righzs reserved.
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