Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 173, Issue -, Pages 765-771Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2012.06.096
Keywords
Boiling water; Hydrophilic surface modification; PDMS; Silicone elastomer; Hydrosilylation; Biocompatibility; Hydroxyl groups
Funding
- National Research Lab (NRL) program
- Korea Science and Engineering Foundation (KOSEF), Republic of Korea [R0A-20110020455]
- National Research Foundation of Korea (NRF)
- Ministry of Education, Science and Technology [2012R1A1A1015181]
- National Research Foundation of Korea [2007-0056875, 2012R1A1A1015181] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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Polydimethylsiloxane (PDMS) lacks cell binding sites and requires additional treatments such as a routine extracellular matrix coating process to culture cells on it. Here we propose a simple method to improve the cytophilicity of hydrosilylation cured PDMS elastomers by using boiling water: placing the crosslinked PDMS substrate in boiling deionized water for about 1 h generates hydroxyl groups on the surface derived from residual SiH functional groups present in the cured elastomers that act as good binding sites for cell attachment. Modified chemistry of the PDMS surface was confirmed by using attenuated total reflection infrared (ATR-IR) analysis. The beneficial biological effects were demonstrated through enhanced attachment/spreading of human mesenchymal stem cells on the boiling-treated surface of PDMS. The results show that boiling water treatment offers a mild, convenient, and cost-effective method to improve the surface cytophilicity of PDMS. (C) 2012 Elsevier B.V. All rights reserved.
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