Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 171, Issue -, Pages 93-101Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2011.07.052
Keywords
Dopamine; Molecularly imprinted polymer; Over-oxidized polypyrrole; Differential normal pulse voltammetry; Parkinson's disease
Funding
- National Health Research Institute of Taiwan [NHRI-EX-99-9835EI]
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A highly sensitive and selective electrochemical microelectrode using the molecularly imprinted (MIP) electropolymer of over-oxidized polypyrrole (OPPy) was proposed for in vivo detection of dopamine (DA) which process is different from current approaches for in vitro DA detection using MIP-based macro-electrode. The proposed MIP electrosynthesized by our present method under a DA/pyrrole-monomer concentration ratio of 1 mM/10 mM yielded the best results for DA adsorption, reaching 2.6-fold that of similar microelectrodes without imprinting. Differential normal pulse voltammetry techniques was used to evaluate the DA-imprinted OPPy microelectrodes for demonstrating high selectivity for in vitro DA detection under conditions with various competitive electroactive molecules. The validity of the modified OPPy-MIP thin-film microelectrodes was further verified with amperometric i-t measurements, which showed a lower DA levels of detection (10-100 nM) and the low detection limit of 4.5 nM with signal to noise ratio of 3. Good reproducibility with relative standard deviation of 5.45% and long-term stability within two weeks were also observed. The developed OPPy-MIP microelectrode has been successfully applied for acute in vivo DA detection in the striatum of anesthetized rats with 3,4-dihydroxy-L-phenylalanine (L-DOPA) administration, which can be used for future evaluation of novel therapeutic treatments in Parkinsonian rats. Crown Copyright (C) 2011 Published by Elsevier B.V. All rights reserved.
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