Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 155, Issue 1, Pages 75-80Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2010.11.026
Keywords
Lactate biosensor; Laponite/chitosan hydrogel; Ferrocene-methanol; Wine; Beer; Dairy products
Funding
- Consejo Nacional de Investigaciones Cientificas y Tecnologicas (CONICET)
- Consejo de Investigaciones Cientificas y Tecnologicas de la Universidad Nacional de Santiago del Estero
- Secretaria de Ciencia y Tecnologia de la Universidad Nacional de Cordoba
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A biosensor based on the immobilization of lactate oxidase (LOx) on a glassy carbon electrode modified with laponite/chitosan hydrogels for the quantification of I.-lactate in alcoholic beverages and dairy products is presented. Ferrocene-methanol (FcMe) is used as artificial mediator. The purpose of this work is to determine the best hydrogel composition from the analytical point of view. The characterization of the hydrogels was carried out by CV, amperometry and EIS. According to permeabilities and charge transfer resistances for ferrocyanide (used as molecular probe) as well as the enzymatic behavior of the enzyme fort-lactate, the best laponite/chitosan mass ratio found was 25/50. The distinct features of the bioelectrode are its long stability, its ability to reject or minimize most interferents including ascorbic acid, and its excellent analytical response, which allowed the reduction of the enzyme content below 0.5 U, for a sensitivity of (0.326 +/- 0.003)A cm(-2) M-1, with a time response lower than 5s and a detection limit of (3.8 +/- 0.2) x 10(-6) M. Our L-lactate biosensor was validated by comparison with a standard spectroscopic method. (C) 2010 Published by Elsevier B.V.
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