Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 155, Issue 2, Pages 545-550Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2011.01.005
Keywords
Biosensor; Cholesterol; Esterase; Dehydrogenase; Redox mediator
Funding
- National High Technology Research and Development Program of China [2007AA022006]
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A disposable amperometric biosensor for detecting the total cholesterol was fabricated which comprises a sensing electrode and a reference electrode in simultaneous contact with an integrated reagent layer. The integrated reagent layer formed by coating a working ink containing cholesterol esterase, cholesterol dehydrogenase, coenzyme, redox mediator, surfactant, stabilizer, filler and at least one aqueous thickening agent. The biosensor showed the linearity for 50-500 mg/dL cholesterol acetate. The minimum detection limit of the cholesterol was 50 mg/dL. The effects of temperature and the stability of immobilized enzymes were also studied. A good correlation was found among cholesterol values obtained by commercial colorimetric test strip and clinical/laboratory methods. The biosensor showed an acceptable reproducibility, good stability and low interferences. (C) 2011 Elsevier B.V. All rights reserved.
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