Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 160, Issue 1, Pages 891-898Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2011.08.078
Keywords
Biosensor; Capacitive transducer; C-reactive protein (CRP); Cardiovascular diseases (CVD); Bovine serum albumin (BSA); Prostate specific antigen (PSA)
Funding
- Scientific and Technological Research Council of Turkey (TUBITAK) [107E014]
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This paper presents, for the first time, the development of a nickel-based, reliable alternative electrode for biosensor applications. The electrodes are formed of nanosized nickel patterned as interdigitated structure to form a capacitive biosensor platform. To test the sensing performance of new biosensing platform, we investigated cardiovascular risk marker (C-reactive protein - CRP) and to check the specificity of the bioassay bovine serum albumin (BSA) and prostate specific antigen (PSA) were employed as controls. The nickel-electrode surface was covalently functionalized and characterized by Fourier transform infrared spectroscopy (FT-IR). The detection and quantification of CRP-antigen was performed by impediometric measurements. A detection model for the new biosensing platform was developed using the spectral impediometric responses and are matched with the specific antibody-antigen interaction-model, using the Cole-Cole model parameters. The results and analysis shown that the nanosized, nickel-based-electrode surface could be a good potential to be a cost effective alternative to the conventional gold electrodes with a comparable sensing performance, as shown for CRP, with a detection limit of 1-250 ng/ml. Furthermore, nano-sized nickel-based capacitive electrodes were fabricated using integrated circuit (IC) fabrication processes, providing compatibility for the integration of electronic circuits for single-chip and lab-on-chip biosensing applications. (C) 2011 Elsevier B.V. All rights reserved.
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