A glucose oxidase immobilization platform for glucose biosensor using ZnO hollow nanospheres

A good route (template-directed synthetic route) for the fabrication of ZnO hollow nanospheres (ZnO-HNSPs) was proposed. ZnO hollow nanosphere is a wonderful platform to immobilize glucose oxidase for glucose biosensor owing to the high specific surface area and high isoelectric point (IEP). Along with nafion and glucose oxidase (GOD), a glucose sensor was designed. Nafion/ZnO-HNSPs/GOD/GCE displays higher catalytic activity toward the glucose oxidation than Nafion/ZnO nano-Flowers/GOD/GCE. Linear response was obtained over a concentration range from 5.0 x 10(-3) mM to 13.15 mM with a detection limit of 1.0 mu M (S/N = 3), and the sensitivity was 65.82 mu A/(mM cm(2)). Satisfyingly, the Nafion/ZnO-HNSPs/GOD/GCE could effectively avoid the interferences from the common interfering species such as uric acid (UA), ascorbic acid (AA), dopamine (DA) and fructose. The Nafion/ZnO-HNSPs/GOD modified electrode allows high sensitivity, excellently selective, stable, and fast amperometric sensing of glucose and thus is promising for the future development of glucose sensors. (C) 2010 Elsevier B.V. All rights reserved.