Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 160, Issue 1, Pages 1063-1069Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2011.09.027
Keywords
AC-electrophoretic deposition; Metalloenzymes; Catalase; H2O2 biosensor
Funding
- KU Leuven [GOA/08/007]
- Belgian Federal Science Policy Office (BELSPO) [P6/17]
- Natural Sciences and Engineering Research Council of Canada
- University of Ontario Institute of technology (Canada)
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The deposition of metalloenzymes on electrodes in their highly active state using electric fields is challenging because the presence of metal cations within the enzyme structure renders the enzyme more fragile and high applied currents or voltages may pull out the metal cations from the enzyme structure and lead to its denaturation. In this study, we demonstrate that catalase, a metalloenzyme with four porphyrin heme Fe(III) groups can be deposited using AC-EPD to yield highly active enzyme layers. Under the optimal deposition conditions of 30 Hz. 160 Vp-p, and 30 min deposition time using an unbalanced triangular waveform, the catalase enzyme electrode had a sensitivity for H2O2 of 32.5 nA/mu M mm(2) at a polarization potential of -0.1 V vs. Ag/AgCl. The sensor has a linear response up to 90 mu M H2O2, a fast response time of 4 s. a detection limit below 1 mu M and a reasonable stability without employing stabilizers or an outer polymer layer. (C) 2011 Elsevier B.V. All rights reserved.
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