Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 152, Issue 1, Pages 88-93Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2010.09.031
Keywords
Papaverine; Poly-o-phenylenediamine; Carbon coated nickel; BSA; Biosensor
Funding
- National Nature Science Foundation of China [21075029]
- Natural Science Foundation of Hubei province [2008CDB034]
- Foundation of Hubei Provincial Department of Education for Young Scholars [Q20081006]
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A biosensor based on bovine serum albumin (BSA) and poly-o-phenylenediamine (PoPD)/carbon-coated nickel (C-Ni) nanobiocomposite film modified electrode has been developed to study the interaction of BSA with papaverine (PAP). The well-dispersed C-Ni nanoparticles were dripped onto the glassy carbon electrode (GCE) surface firstly, and PoPD films were subsequently electropolymerized by cyclic voltammetry (CV) to prepare PoPD/C-Ni/GCE. Finally, the BSA was easily immobilized on the PoPD films via electrostatic adsorption. The morphology and the electrochemical properties of the fabricated composite electrodes were examined by scanning electron microscope (SEM) and electrochemical impedance spectroscopy (EIS), respectively. The interaction of PAP with BSA was monitored by differential pulse voltammetry (DPV), using PoPD as the electrochemical indicator. The binding constant (K), obtained by DPV, was 1.7 x 10(4) L/mol, which was consistent with the fluorescence analysis. This constructed biosensor also exhibited a fine linear correlation with PAP concentration range of 2.5 x 10(-9)-4.5 x 10(-5) mol/L and a detection limit of 8.3 x 10(-10) mol/L was achieved by DPV. (c) 2010 Elsevier B.V. All rights reserved.
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