Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 148, Issue 2, Pages 504-510Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2010.05.032
Keywords
Micropatterned scaffolds; Hydrogel microstructures; Electrospun fibers; Cellular micropatterns; Cell-based biosensor
Funding
- Ministry of Education, Science and Technology [2009-0084190, R11-2007-050-03002-0]
- Ministry of Knowledge Economy, Republic of Korea
- Seoul Research and Business Development Program [10816]
- Korea Evaluation Institute of Industrial Technology (KEIT) [K0006005] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
- National Research Foundation of Korea [2007-2004045] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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In this paper, we describe a simple method for fabricating micropatterned polymeric fibrous scaffolds that are capable of controlling the spatial positioning of mammalian cells. Micropatterned scaffolds were prepared by fabricating poly(ethylene glycol) (PEG) hydrogel microstructures on electrospun poly(styrene) (PS) fiber matrices, and produced as free-standing and bidirectionally-porous sheets. Clearly defined hydrogel micropatterns incorporating PS fibers were created using photolithography without any residual hydrogel precursor solution remaining in the PS fiber region. Most of the PS fibers were inserted through the side walls of the hydrogel microstructures, leaving no fiber residue on the top surfaces of hydrogel micropatterns. Since the PEG hydrogel showed non-adhesiveness toward proteins and cells, cells selectively adhered and remained viable within the PS fiber region, thereby creating cellular micropatterns. Finally, possible application of this system to cell-based biosensor was demonstrated using cytotoxicity assay. (C) 2010 Elsevier B.V. All rights reserved.
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