Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 147, Issue 1, Pages 298-303Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2010.03.027
Keywords
cTnI; Colorimetric detection; Silver enhancement; Gold nanoparticles; Biosensor
Funding
- National Natural Science Foundation of China [20635020, 20875080]
- National 863 program of China [2007AA022001]
- China Postdoctoral Science Foundation [200801372, 20070420982]
- Jiangsu Postdoctoral Science Foundation [070201913]
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This paper described poly(dimethylsiloxane) (PDMS)-gold nanoparticles (AuNPs) composite film as basis with silver enhancement for colorimetric detection of cardiac troponin I (cTnI). Stable PDMS-AuNPs composite film was simply prepared with dropping HAuCl4 solution in PDMS microchip, gold nanoparticles were both used as nuclei for silver enhancement anti as basis for adsorption of antibody, immunoassay was implemented after BSA blocking. Because of the inhibition ability difference for silver enhancement between BSA and antibody-antigen complex, darkness of silver deposition was relative to the amount of antigen. The proposed colorimetric method was applied for clinical sample cTnI detection, results were consistent with that from enzyme-linked immunosorbent assay (ELISA). The composite film could be coupled with digital transmission of images for remote monitoring system in diagnosis, food control, and environmental analysis. (c) 2010 Elsevier B.V. All rights reserved.
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