Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 149, Issue 1, Pages 221-225Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2010.05.047
Keywords
Magnetic bead; Electrochemiluminescence; DNA biosensor; Genetically modified organism
Funding
- National Natural Science Foundation of China [30600128]
- Natural Science Foundation of Guangdong Province [7005825]
- Program for Changjiang Scholars and Innovative Research Team in University [IRT0829]
- NSFC-Guangdong joint Funds of China [U0931005]
- National Basic Research Program of China [2010CB732602]
- National High Technology Research and Development Program of China (863 Program) [2007AA10Z204]
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A reusable magnetic bead-based electrochemiluminescence (ECL) DNA biosensor for the detection of genetically modified organism (GMO) is proposed. The sensor method consists of immobilization of biotin-probe using streptavidin-coated magnetic beads, hybridization of target DNA with biotin-probe and ruthenium(II) tris-bipyridal (TBR)-probe, detection of target DNA by direct measuring the ECL emission of TBR. The sensor was applied to detect GMO in engineered tobacco samples. Results indicate that the sensitivity of this sensor method was 5 nmol/L. of CaMV355 DNA. A stable calibration curve with a wide dynamic range was established. The calibration curve was linear from 5 nmol/L to 5 mu mol/L, thus, made quantitative analysis possible. GM tobacco samples and non-GM tobacco samples were clearly discriminated by the sensor method. Results of the study suggest that the reusable DNA biosensor is a feasible tool for daily GMO detection due to its rapidness, simplicity, safety, sensitivity, reliability and low cost. (C) 2010 Elsevier B.V. All rights reserved.
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