Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 141, Issue 2, Pages 592-598Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2009.06.016
Keywords
Glucose biosensor; Platinum nanoparticle; Carbon nanotube; Glucose oxidase; Adsorption
Funding
- National Science Council, Taiwan
- Ministry of Education, Taiwan
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Glucose oxidase (GOx) has been immobilized in platinum-multiwalled carbon nanotube-alumina-coated silica (Pt-MWCNT-ACS) nanocomposite modified glassy carbon electrode by adsorption to provide a novel amperometric glucose biosensor. The morphology, nature, and performance of the resulting GCx-Pt-MWCNT-ACS nanobiocomposite modified glassy carbon electrode were characterized by field emission scanning electron microscopy, energy dispersive X-ray spectroscopy, cyclic voltammetry, and amperometry. The influence of various experimental conditions was examined for the determination of the optimum analytical performance. The optimized glucose biosensor displayed a wide linear range of up to 10.5 mM, a high sensitivity of 113.13 mA M-1 cm(-1), and a response time of less than 5 s. The sensitivity for the determination of glucose at the GOx-Pt-MWCNT-ACS nanobiocomposite modified glassy carbon electrode is better than at common GOx-Pt-CNT nanobiocomposite modified electrodes. The proposed biosensor has good anti-interferent ability and long-term storage stability after coating with Nafion, and it can be used for the determination of glucose in synthetic serum. (C) 2009 Elsevier B.V. All rights reserved.
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