Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 136, Issue 2, Pages 516-522Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2008.09.020
Keywords
Conductive polymers; Hydrogel; Glucose oxidase; Alginate-pyrrole; Biosensor
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The aim of this paper is to report the characterization and application of a new and attractive hybrid molecule (alginate modified with pyrrole) alginate-pyrrole (Alg-py) with glucose oxidase (GOx) to create a highly responsive glucose biosensor. The biosensor was built by covalent attachment of GOx with the Alg-py matrix, followed by pyrrole polymerization, then casting the biocomposite onto the surface of a platinum electrode (Pt). The unique three-dimensional structure, porous morphology, conductive setup, mechanical firmness and biocompatibility resulted in low response time (4s), high sensitivity value (16 mu A mM(-1) cm(-2)) and a very low enzyme load (9.2 U). (C) 2008 Elsevier B.V. All rights reserved
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