Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 130, Issue 1, Pages 120-128Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2007.07.136
Keywords
surface plasmon resonance (SPR); tetrodotoxin (TTX); pufferfish; neurotoxin; seafood toxin; antibody inhibition assay
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We report the quantitative antibody-based detection of a low-molecular weight molecule tetrodotoxin (TTX) (similar to 319 Da) by inhibition assay with a surface plasmon resonance (SPR) sensor. A novel anti-TTX antibody sensing surface was developed by chemically immobilizing TTX on a gold film with a mixed self assembled monolayer (SAM) consisting of amine terminated oligo-ethylene glycol (OEG) alkanethiol and a hydroxyl terminated OEG alkanethiol. The ratio of amine to hydroxyl terminated OEG alkanethiols and TTX immobilization chemistry were optimized to maximize specific anti-TTX binding, while minimizing non-specific binding. The calibration curves are reported for two antibody concentrations incubated with samples of varying TTX concentrations ranging from 0.01 to 10,000 ng/mL. The detection limit for TTX is defined as IC20 (20% inhibitory concentration), which is similar to 0.3 ng/mL in this work. The corresponding calibration curve has a characteristic IC50 (50% inhibitory concentration) of similar to 6 ng/mL. The ability to reproducibly regenerate the TTX-immobilized surface was also demonstrated. (C) 2007 Elsevier B.V. All rights reserved.
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