Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 134, Issue 1, Pages 182-188Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2008.04.026
Keywords
Horseradish peroxidase; Direct electrochemistry; Biosensor; Manganese oxide nanosheets; Polyquaternium
Funding
- National Natural Science Foundation of China
- National 863 Project [2006AA03Z343]
- the 111 Project [B07004]
- Program for Changjiang Scholars and Innovative Research Teams in Universities [IRT0406]
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A novel biocompatible polyquaternium (QY)-manganese oxide nanosheet (MNS) nanocomposite has been prepared and shown to be a promising matrix for horseradish peroxidase (HRP) immobilization. The resulting HRP-QY-MNS film was characterized by Fourier transform infrared (FTIR) and circular dichroism (CD) spectroscopy, which indicated that HRP retained its native structure in the nanocomposite film. An HRP-QY-MNS film-modified glassy carbon electrode exhibited a pair of well-defined and quasi-reversible cyclic voltammetric peaks centered at -0.272 V (vs. Ag/AgCl) in pH 7.0 phosphate buffer solution. The direct electrochemical behavior of HRP was greatly enhanced in the QY-MNS nanocomposite film compared with that in single-component QY or MNS films. The immobilized HRP showed excellent electrocatalysis in the reduction of hydrogen peroxide (H2O2), which was exploited in the construction of an H2O2 biosensor. The linear range of the biosensor for H2O2 was found to be from 1.0 x 10(-7) to 3.2 x 10(-5) M with a correlation coefficient of 0.998. The detection limit was 7.8 x 10(-8) M at a signal-to-noise ratio of 3. The biosensor exhibited rapid response and good long-term stability. (C) 2008 Elsevier B.V. All rights reserved.
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