Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 132, Issue 2, Pages 361-367Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2007.09.076
Keywords
beads; electrostatic self-assembly; streptavidin; micropatterned magnetic beads
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We demonstrate a simple and fast method for sef-assembly-driven micropatterning of protein-functionali zed magnetic beads on glass. We use positively charged aminosilane micro-patterns as template for selective immobilization of streptavidin-coated beads by electrostatic interactions. We show that addition of a non-ionic surfactant to the bead solution increases the selectivity of the micropatterning process. Streptavidin-coated magnetic beads (1.05 and 2.8 mu m in size) are immobilized, with high reproducibility and in a very short processing time (similar to 30 min), in the form of stripes and dots, both on bare substrates and in situ in microfluidic channels. The arrangement and the number of immobilized beads can be controlled by tuning the aminosilane template size. (c) 2007 Elsevier B.V. All rights reserved.
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