Micropatterning of protein-functionalized magnetic beads on glass using electrostatic self-assembly

We demonstrate a simple and fast method for sef-assembly-driven micropatterning of protein-functionali zed magnetic beads on glass. We use positively charged aminosilane micro-patterns as template for selective immobilization of streptavidin-coated beads by electrostatic interactions. We show that addition of a non-ionic surfactant to the bead solution increases the selectivity of the micropatterning process. Streptavidin-coated magnetic beads (1.05 and 2.8 mu m in size) are immobilized, with high reproducibility and in a very short processing time (similar to 30 min), in the form of stripes and dots, both on bare substrates and in situ in microfluidic channels. The arrangement and the number of immobilized beads can be controlled by tuning the aminosilane template size. (c) 2007 Elsevier B.V. All rights reserved.