Journal
SENSORS
Volume 18, Issue 8, Pages -Publisher
MDPI
DOI: 10.3390/s18082607
Keywords
urea biosensor; silk fibroin; amination; flow conditions; real-time monitoring
Funding
- National Research Foundation of Korea (NRF) - Korea government (MSIP) [NRF-2015R1A4A1041631]
- Korea Health Technology, R&D Project, Ministry of Health & Welfare, Republic of Korea [HI14C0559]
- Korea Health Promotion Institute [HI14C0559040018] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
- National Research Foundation of Korea [2015R1A4A1041631] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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In this study, a portable urea sensor that monitors the urea concentration in flow conditions was fabricated. We propose an electrochemical sensor that continually measures the urea concentration of samples flowing through it at a constant flow rate in real time. For the electrochemical sensing, a porous silk fibroin membrane with immobilized urease was mounted in a polydimethylsiloxane (PDMS) sensor housing. The fabricated urea sensor elicited linear current-concentration characteristics in the clinically significant concentration range (0.1-20 mM) based on peritoneal dialysis. The sensor maintained the linear current-concentration characteristics during operation in flow conditions.
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