4.6 Article

Development of an Immunochromatographic Strip Test for Rapid Detection of Ciprofloxacin in Milk Samples

Journal

SENSORS
Volume 14, Issue 9, Pages 16785-16798

Publisher

MDPI
DOI: 10.3390/s140916785

Keywords

ciprofloxacin; immunochromatographic assay; test strip; monoclonal antibody

Funding

  1. MOST [2012BAK08B01, 2012BAD29B04]
  2. Jiangsu Province, MOF
  3. MOE [NCET-12-0879, BE2013613, 201310135]

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A rapid, simple, and sensitive immunochromatographic test strip has been developed for testing residues of ciprofloxacin (CIP). A specific and sensitive monoclonal antibody (mAb) for CIP was generated by immunizing BALB/c mice with well-characterized CIP-Keyhole limpet haemocyanin. Under the optimized conditions, the cut-off limits of test strips for CIP were found to be 5 ng/mL in phosphate-buffered saline and 2.5 ng/mL in milk samples. Each test can be evaluated within 3 min. The cross-reactivities of the CIP test strip to enrofloxacin (ENR), norfloxacin (NOR), nadifloxacin (NDF), danofloxacin (DANO), pefloxacin (PEX), lomefloxacin (LOME), enoxacin (ENO), and sarafloxacin (SAR) were 71.4%, 71.4%, 66%, 50%, 33%, 20%, 12.5%, and 6.25%, respectively. The data indicate that the method is sensitive, specific, and has the advantages of simplicity and speed, therefore, this test strip is a useful screening method for the detection of CIP residues in milk samples.

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