Journal
SENSORS
Volume 13, Issue 2, Pages 2011-2022Publisher
MDPI AG
DOI: 10.3390/s130202011
Keywords
surface electromagnetic waves; label-free sensing; fluorescence; multilayers; bragg mirrors
Funding
- Piedmont regional project CIPE PHOENICS
- Italian FIRB NEWTON [RBAP11BYNP]
- European Union Seventh Framework Programme (FP7) [318035]
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A one-dimensional photonic crystal (1DPC) based on a planar stack of dielectric layers is used as an optical transducer for biosensing, upon the coupling of TE-polarized Bloch Surface Waves (BSW). The structure is tailored with a polymeric layer providing a chemical functionality facilitating the covalent binding of orienting proteins needed for a subsequent grafting of antibodies in an immunoassay detection scheme. The polymeric layer is impregnated with Cy3 dye, in such a way that the photonic structure can exhibit an emissive behavior. The BSW-coupled fluorescence shift is used as a means for detecting refractive index variations occurring at the 1DPC surface, according to a label-free concept. The proposed working principle is successfully demonstrated in real-time tracking of protein G covalent binding on the 1DPC surface within a fluidic cell.
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