Journal
SENSORS
Volume 13, Issue 6, Pages 7774-7785Publisher
MDPI
DOI: 10.3390/s130607774
Keywords
biosensor; impedance; membrane; nanochannel; nanopore; dengue; infectious disease
Funding
- NTU
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A novel and integrated membrane sensing platform for DNA detection is developed based on an anodic aluminum oxide (AAO) membrane. Platinum electrodes (similar to 50-100 nm thick) are coated directly on both sides of the alumina membrane to eliminate the solution resistance outside the nanopores. The electrochemical impedance technique is employed to monitor the impedance changes within the nanopores upon DNA binding. Pore resistance (R-p) linearly increases in response towards the increasing concentration of the target DNA in the range of 1 x 10(-12) to 1 x 10(-6) M. Moreover, the biosensor selectively differentiates the complementary sequence from single base mismatched (MM-1) strands and non-complementary strands. This study reveals a simple, selective and sensitive method to fabricate a label-free DNA biosensor.
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